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An active Ac-like transposable element in teleost fish

Hori1, Suzuki, Inagaki

  • 1Department of Biology, Faculty of Science, Nagoya University, Nagoya 464-8602, Japan

Journal of Marine Biotechnology
|May 18, 1999
PubMed
Summary
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A novel transposable element, Tol2, inserted into the medaka fish tyrosinase gene causes a quasi-albino phenotype. This active Ac-like element has potential applications for gene tagging systems in fish.

Area of Science:

  • Genetics
  • Molecular Biology
  • Ichthyology

Background:

  • The i4/i4 genotype in medakafish (Oryzias latipes) displays a quasi-albino phenotype.
  • This phenotype is linked to the insertion of a novel transposable element into the tyrosinase gene.

Purpose of the Study:

  • To characterize the novel transposable element, Tol2, responsible for the quasi-albino phenotype in medakafish.
  • To investigate the molecular properties and potential applications of Tol2.

Main Methods:

  • Molecular characterization of the Tol2 element, including size, terminal repeats, and Open Reading Frames (ORFs).
  • Polymerase Chain Reaction (PCR) analysis to detect excision activity.
  • Genomic Southern blotting to determine copy number.
  • Amino acid sequence comparison of Tol2 ORFs with known transposases.

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Main Results:

  • Tol2 is a 4681 bp element with inverted terminal repeats and four ORFs, potentially encoding a transposase.
  • Excision activity of Tol2 was confirmed via PCR.
  • Approximately 20 copies of Tol2 are present in the medaka diploid genome.
  • Tol2 ORFs show significant amino acid similarity to active transposases from other species (e.g., Ac, hobo, Tam3).

Conclusions:

  • Tol2 is identified as an active Ac-like transposable element in medakafish.
  • The element likely encodes a functional transposase protein.
  • Tol2 represents a valuable tool for developing gene tagging systems in fish genetics research.