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Related Experiment Videos

Selection system for genes encoding nuclear-targeted proteins

N Ueki1, T Oda, M Kondo

  • 1Biological Technology Laboratory, Helix Research Institute, Chiba, Japan. goose@rc.m-kagaku.co.jp

Nature Biotechnology
|December 16, 1998
PubMed
Summary

Researchers developed a novel yeast selection system, the nuclear transportation trap (NTT), to efficiently identify genes encoding nuclear transport signals and novel nuclear proteins for cell function annotation.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Nuclear proteins are crucial for cell proliferation and differentiation.
  • Understanding nuclear protein localization is key to deciphering cellular functions.
  • Existing methods for identifying nuclear transport signals can be limited.

Purpose of the Study:

  • To develop and validate a novel yeast selection system, the nuclear transportation trap (NTT).
  • To identify genes encoding nuclear transport signals from a human fetal brain cDNA library.
  • To assess the efficiency of NTT in isolating nuclear-targeted proteins.

Main Methods:

  • Development of the nuclear transportation trap (NTT) yeast selection system.
  • Screening of a human fetal brain cDNA library using the NTT system.

Related Experiment Videos

  • Validation of identified nuclear localization signals in COS-7 cells.

    • Main Results:

    • The NTT system successfully identified both known and novel nuclear localization signals.
    • A significant portion (75%) of newly identified proteins were exclusively localized to the nucleus.
    • The system demonstrated efficiency in isolating cDNAs encoding nuclear-targeted proteins.

    Conclusions:

    • The nuclear transportation trap (NTT) is an effective method for isolating cDNAs encoding nuclear-targeted proteins.
    • NTT facilitates the discovery of novel nuclear proteins.
    • This system aids in the functional annotation of genes involved in nuclear transport.