Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Activity-dependent modulation of synaptic AMPA receptor accumulation

R J O'Brien1, S Kamboj, M D Ehlers

  • 1Howard Hughes Medical Institute, Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Neuron
|December 18, 1998
PubMed
Summary

Central neurons adjust excitatory synaptic input by regulating AMPA receptors. Inhibiting transmission increases synaptic AMPA receptors and mEPSC amplitudes, while increasing activity decreases them, indicating activity-dependent receptor turnover.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Midlife adiposity predicts earlier onset of Alzheimer's dementia, neuropathology and presymptomatic cerebral amyloid accumulation.

Molecular psychiatry·2023
Same author

Technological advancements in jaggery-making processes and emission reduction potential via clean combustion for sustainable jaggery production: An overview.

Journal of environmental management·2021
Same author

Inflammation-induced GluA1 trafficking and membrane insertion of Ca<sup>2+</sup> permeable AMPA receptors in dorsal horn neurons is dependent on spinal tumor necrosis factor, PI3 kinase and protein kinase A.

Experimental neurology·2017
Same author

Enhancing VTA Ca<sub>v</sub>1.3 L-type Ca<sup>2+</sup> channel activity promotes cocaine and mood-related behaviors via overlapping AMPA receptor mechanisms in the nucleus accumbens.

Molecular psychiatry·2017
Same author

Alpha-2 macroglobulin in Alzheimer's disease: a marker of neuronal injury through the RCAN1 pathway.

Molecular psychiatry·2016
Same author

The Mind Bending Quest for Cognitive Enhancers.

Clinical pharmacology and therapeutics·2016

Area of Science:

  • Neuroscience
  • Cellular Neuroscience
  • Synaptic Plasticity

Background:

  • Central neurons exhibit homeostatic plasticity to maintain stable output despite fluctuating synaptic input.
  • Understanding the mechanisms of synaptic scaling is crucial for comprehending neuronal network function.

Purpose of the Study:

  • To investigate how excitatory synaptic transmission influences the number and function of AMPA receptors at synapses.
  • To elucidate the role of AMPA receptor turnover in synaptic homeostasis.

Main Methods:

  • Utilized cultured spinal neurons to study synaptic plasticity.
  • Manipulated excitatory synaptic transmission to observe changes in AMPA receptor dynamics.
  • Measured miniature excitatory postsynaptic current (mEPSC) amplitudes and AMPA receptor accumulation at synapses.

Related Experiment Videos

Main Results:

  • Inhibition of excitatory transmission increased mEPSC amplitudes and synaptic AMPA receptor accumulation.
  • Increased excitatory activity decreased synaptic AMPA receptors and mEPSC amplitudes.
  • Synaptic remodeling occurred slowly, correlating with AMPA receptor metabolic half-life.
  • Reduced transmission prolonged the half-life of the GluR1 AMPA receptor subunit.

Conclusions:

  • Synaptic activity dynamically regulates the number of postsynaptic AMPA receptors.
  • Neuronal activity modulates the size of mEPSCs by controlling AMPA receptor turnover.
  • This provides a mechanism for synaptic homeostasis in central neurons.