A method for endoscopic embryo collection and transfer in the rabbit

Area of Science:

• Reproductive Biology
• Veterinary Science
• Endoscopic Surgery

Background:

• Assisted reproductive technologies are crucial for conservation and research.
• Efficient embryo collection and transfer methods are vital for maximizing success rates.
• Endoscopic techniques offer minimally invasive options for reproductive procedures.

Purpose of the Study:

• To evaluate the efficacy of combined endoscopic techniques for embryo collection and transfer in rabbits.
• To determine the reproductive outcomes following transcervical embryo transfer under laparoscopic guidance.

Main Methods:

• Thirty-two rabbits were utilized for the study.
• Embryo collection involved a combination of midventral laparoscopy and transcervical endoscopy for orthograde flushing.
• Embryo transfer was performed transcervically with laparoscopic control.

Main Results:

• A mean of 13.3 corpora lutea were observed in nine donor rabbits.
• 72 morulae/blastocysts were successfully collected; flushing only uterine horns yielded no embryos.
• 291 embryos were transferred to 23 recipients, resulting in 10 pregnancies at day 12.
• Four recipients delivered a total of 12 pups, with 4 implantation sites noted in two slaughtered recipients.

Conclusions:

• Combined endoscopic procedures are effective for rabbit embryo collection.
• Transcervical embryo transfer under laparoscopic control can lead to successful pregnancies and live offspring in rabbits.
• This minimally invasive approach shows promise for improving assisted reproduction outcomes in rabbits.