[Characteristics of different sublines of VNK-21 cells]

Comparative cytological, histological, histochemical and partially karyological studies were carried out on 8 batches of BHK-21 cell kept at different conditons. It was found that cells adapted to suspension culture differed from monolayer culture cells both morphologically and cytochemically. Suspension cells transmitted into monolayer culture displayed smaller size, changeable form, increased glycogen accumulation, decreased enzymatic activity (acid phosphatase, succinate dehydrogenase), their karyotypes tending to hypodiploidy.