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Terbium chelates for fluorescence immunoassays
1A.V. Bogatsky Physico-Chemical Institute of Academy of Sciences of Ukraine, Odessa.
Journal of Pharmaceutical and Biomedical Analysis
|December 24, 1998
Summary
This study shows how terbium(III) ion luminescence is enhanced by oxolinic acid. The developed method achieves high sensitivity for terbium detection, suitable for time-resolved fluoroimmunoassays.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Materials Science
Background:
- Terbium(III) ion luminescence is a valuable tool in bioassays.
- Oxolinic acid can potentially sensitize lanthanide luminescence.
- Antibody-based assays require sensitive detection methods.
Purpose of the Study:
- To investigate the sensitization of terbium(III) ion luminescence by oxolinic acid.
- To develop a sensitive detection method for terbium(III) in antibody conjugates.
- To evaluate the suitability of this system for time-resolved fluoroimmunoassays.
Main Methods:
- Terbium(III) labeling of antibodies using diethylenetriamine-pentaacetic acid anhydride (DTPAA).
- Luminescence measurements of terbium(III)-DTPAA-antibody conjugates in the presence of oxolinic acid.
- Optimization of pH and surfactant conditions for luminescence intensity.
- Determination of detection sensitivity.
Main Results:
- Oxolinic acid significantly sensitizes terbium(III) ion luminescence.
- Optimum luminescence intensity is achieved at pH 7.5.
- Cationic surfactant (cetyltrimethylammonium bromide) further enhances luminescence.
- High sensitivity for terbium(III) detection (5 x 10^-14 mol L^-1) was achieved.
Conclusions:
- The terbium(III)-oxolinic acid luminescence system offers enhanced sensitivity.
- This system is a promising candidate for time-resolved fluoroimmunoassays.
- The method provides a sensitive approach for detecting antibody-bound terbium(III).