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Centrifugal pulsing increases the recovery of viable spermatozoa
P Bielfeld1, V Acosta, R S Jeyendran
1Department of Obstetrics and Gynecology, Heinrich Heine University, Dusseldorf, Germany.
Purpose:
Our purpose was to compare the number and quality of spermatozoa recover following the standard centrifugation method with those after pulsing the relative centrifugal field during centrifugation.
Methods:
A prospectively controlled in vitro study using ejaculates from 10 healthy sperm donors was undertaken. Number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5 degrees C in TEST-yolk results determined the efficacy of the centrifugation techniques.
Results:
The number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5 degrees C in TEST-yolk were significantly higher for the sperm recovered following pulse centrifugation compared with those after standard centrifugation.
Conclusions:
Pulsing the relative centrifugal field during centrifugation yields a higher number of viable spermatozoa.