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On-line dialysis solid-phase extraction coupled to capillary electrophoresis

J R Veraart1, J van Hekezen, M C Groot

  • 1Vrije Universiteit, Department of Analytical Chemistry, Amsterdam, The Netherlands.

Electrophoresis
|December 31, 1998
PubMed
Summary

This study introduces an automated dialysis solid-phase extraction (SPE) coupled with capillary electrophoresis (CE) for analyzing sulfonamides in biological samples. The novel method efficiently prepares samples and achieves sensitive detection in serum and urine.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Sulfonamide analysis in biological matrices requires efficient sample preparation to remove interfering substances like proteins and salts.
  • Existing methods can be time-consuming and prone to clogging, necessitating robust and automated solutions.

Purpose of the Study:

  • To develop and validate a fully automated on-line dialysis solid-phase extraction (SPE) coupled with capillary electrophoresis (CE) system.
  • To determine sulfonamides in serum and urine samples using the developed automated system.

Main Methods:

  • An integrated system combining dialysis for protein removal and SPE for analyte reconcentration and interference removal was designed.
  • Analytes were desorbed and injected into a CE system via a custom-built interface.
  • The system was validated for sensitivity, precision, and long-term stability.

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Main Results:

  • Achieved limits of detection (LOD) ranging from 0.05 to 0.1 microg/mL in urine and 0.05 to 0.3 microg/mL in serum.
  • Demonstrated excellent within-day (2-6%) and between-day (3-8%) precision at five times the LOD.
  • Successfully analyzed over 500 serum and urine samples over six months without system clogging.

Conclusions:

  • The automated dialysis SPE-CE system offers a robust, sensitive, and efficient method for sulfonamide determination in complex biological samples.
  • This on-line approach minimizes manual intervention and enhances analytical throughput.
  • The system's stability and reliability make it suitable for routine clinical and research applications.