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Organometallic photonucleases: a novel class of DNA-cleaving agents
D L Mohler1, D R Dain, A D Kerekes
1Department of Chemistry, West Virginia University, Morgantown 26506-6045, USA.
Bioorganic & Medicinal Chemistry Letters
|January 1, 1999
Abstract:
The first demonstration of DNA cleavage by an organic radical generated via homolysis of a metal-alkyl bond in a Cp-metal complex is presented. Irradiation of CpW(CO)3CH3 (1.5 molecules/base pair) produced methyl radical, giving single-strand cleavage of pBR322 DNA. This process was inhibited by the general radical trap cysteine and by TEMPO, which traps carbon radicals but not oxygen-centered radicals.