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Updated: Aug 14, 2026

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
Published on: November 26, 2014
Hyperthermophilic redox chemistry: a re-evaluation
P L Hagedoorn1, M C Driessen, M van den Bosch
1Wageningen University and Research Centre, Department of Biomolecular Sciences, The Netherlands. peter.leonhagedoorn@epr.bc.wau.nl
Abstract:
The redox chemistry of Pyrococcus furiosus rubredoxin and ferredoxin has been studied as a function of temperature in direct voltammetry and in EPR monitored bulk titrations. The Ems of both proteins, measured with direct voltammetry, have a normal (linear) temperature dependence and show no pH dependence. EPR monitoring is not a reliable method to determine the temperature dependence of the Em: upon rapid freezing the proteins take their conformation corresponding to the freezing point of the solution.
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