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On the preparative use of recombinant pig alpha(1-3)galactosyl-transferase
G Baisch1, R Ohrlein, F Kolbinger
1Novartis Pharma AG, Basle, Switzerland.
Bioorganic & Medicinal Chemistry Letters
|January 5, 1999
Abstract:
A series of non-natural N-acyl derivatives of lactosamine is incubated with recombinant alpha(1-3)galactosyl-transferase and UDP-galactose. The enzyme shows a high promiscuity towards the non-natural acceptors. It selectively transfers a galactose unit onto the 3-OH group of the terminal beta-linked galactose in an alpha-mode to give an array of linear-B trisaccharides.