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A simple procedure for solid-phase synthesis of peptide nucleic acids with N-terminal cysteine
T E Goodwin1, R D Holland, J O Lay
1Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Bioorganic & Medicinal Chemistry Letters
|January 5, 1999
Abstract:
Problems were encountered during attempts to prepare N-terminal cysteine-substituted peptide nucleic acids (PNAs) from commercially available, Fmoc-protected monomers. These problems have been surmounted by the use of an S-t-butylmercapto protecting group on the cysteine moiety. The solid-phase syntheses are carried out via a simplified procedure which should be generally useful for manual PNA synthesis.