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Related Experiment Videos

Induced cell trauma during in vitro perfusion: a comparison between two different perfusion systems

M Skogby1, K Mellgren, K Adrian

  • 1Department of Anesthesiology, Sahlgrenska University Hospital, Göteborg, Sweden.

Artificial Organs
|January 6, 1999
PubMed
Summary

The AREC system showed lower platelet and neutrophil activation compared to the centrifugal pump system during in vitro extracorporeal membrane oxygenation (ECMO). Hemolysis was also reduced with the AREC system, suggesting improved biocompatibility.

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Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Hematology

Background:

  • Extracorporeal membrane oxygenation (ECMO) involves prolonged blood contact with artificial surfaces.
  • Blood-cell activation and damage are critical concerns during ECMO, impacting patient outcomes.
  • Different ECMO system components may influence blood compatibility.

Purpose of the Study:

  • To compare blood cell activation and hemolysis between two distinct in vitro ECMO perfusion systems.
  • To evaluate the biocompatibility of an assistance respiratoire extra corporelle (AREC) system versus a centrifugal pump system.

Main Methods:

  • In vitro long-term perfusion of two ECMO systems: AREC (silicon tubing, hollow-fiber oxygenator) and centrifugal pump (PVC tubing, membrane oxygenator).
  • Assessment of platelet activation via membrane glycoprotein expression (GPIb, GPIIb/IIIa) and plasma beta-thromboglobulin (beta-TG).

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  • Evaluation of neutrophil activation via CD11b expression and measurement of plasma cytokines (IL-1beta, IL-6, IL-8) and plasma hemoglobin for hemolysis.

    • Main Results:

    • The AREC system demonstrated significantly lower platelet activation (GPIb, GPIIb/IIIa expression).
    • Neutrophil activation (CD11b expression) was significantly lower in the AREC system.
    • The AREC system exhibited significantly reduced hemolysis (plasma hemoglobin).
    • No significant differences in IL-1beta, IL-6, or IL-8 concentrations were observed between systems.

    Conclusions:

    • The AREC system shows superior biocompatibility compared to the centrifugal pump system in vitro.
    • Reduced platelet and neutrophil activation, along with lower hemolysis, suggest the AREC system may offer improved hemocompatocompatibility during ECMO.
    • Further research is needed to identify specific components responsible for these differences.