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Developmental changes in Asn-linked neutral oligosaccharides in murine cerebrum
Y Yoshimi1, S Yamazaki, M Ikekita
1Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda, Chiba, Japan. masalab@rs.noda.sut.ac.jp
Biochimica Et Biophysica Acta
|January 8, 1999
Summary
During murine brain development, specific changes in Asn-linked oligosaccharides were observed. Oligosaccharide I decreased while oligosaccharide II increased, offering insights into central nervous system development.
Area of Science:
- Neuroscience
- Glycobiology
- Biochemistry
Background:
- Asn-linked oligosaccharides play crucial roles in biological processes.
- Understanding their developmental changes in the brain is vital for neuroscience research.
Purpose of the Study:
- To investigate the developmental changes in Asn-linked oligosaccharide composition in the murine cerebrum.
- To characterize the structures of identified oligosaccharides and their developmental expression patterns.
Main Methods:
- High-performance liquid chromatography (HPLC) for oligosaccharide separation.
- Electrospray ionization mass spectrometry (ESI-MS) for structural analysis.
- Sequential exoglycosidase digestions to determine oligosaccharide types.
Main Results:
- Two distinct Asn-linked oligosaccharides, designated I and II, showed altered expression during postnatal development.
- Oligosaccharide I, an oligomannose type, decreased during brain development.
- Oligosaccharide II, a biantennary complex type with fucose, increased during development.
Conclusions:
- Asn-linked oligosaccharide composition undergoes significant changes during murine brain development.
- These findings provide crucial insights into the role of specific glycans in central nervous system development.