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Generation of carboxy-terminally deleted forms of STAT5 during preparation of cell extracts
K Garimorth1, T Welte, W Doppler
1Institut für Medizinische Chemie und Biochemie, Universität Innsbruck, Fritz Pregl-Strasse 3, Innsbruck, A-6020, Austria.
Experimental Cell Research
|January 12, 1999
Abstract:
Carboxy-terminally deleted forms of STAT5 have been described to be generated in vivo either by proteolytic processing or by differential splicing mechanisms. By comparing two different cell extraction procedures, we can show that in the mammary gland carboxy-terminally deleted forms are produced in vitro and are not detectable in extracts prepared by SDS lysis.
