Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tau expression in denervated rat muscles

S I Nagao1, T Kumamoto, T Masuda

  • 1Third Department of Internal Medicine, Oita Medical University, Japan.

Muscle & Nerve
|January 12, 1999
PubMed
Summary

Denervation increases phosphorylated tau protein in rat soleus muscles. This tau accumulation, along with beta-tubulin, may help maintain muscle fiber integrity during atrophy or regeneration.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel surgical strategy for the resection of duodenal gastrointestinal stromal tumours located close to the duodenal ampulla: a case report.

Annals of the Royal College of Surgeons of England·2019
Same author

Comparison of a vitamin E-infused highly crosslinked polyethylene insert and a conventional polyethylene insert for primary total knee arthroplasty at two years postoperatively.

The bone & joint journal·2019
Same author

Inferior pancreatic approach for laparoscopic splenic flexure mobilization.

Techniques in coloproctology·2017
Same author

Severe Sepsis After Living Donor Liver Transplantation: Risk Factors and Outcomes.

Transplantation proceedings·2016
Same author

Difference in efficacy of proton pump inhibitor between new-onset and recurrent gastroesophageal reflux disease: Result from a study of on-demand versus continuous maintenance therapy in Japan.

Hippokratia·2015
Same author

Evaluation of nanodispersion of iron oxides using various polymers.

Indian journal of pharmaceutical sciences·2014

Area of Science:

  • Muscle physiology
  • Cellular biology
  • Neuroscience

Background:

  • Tau protein is crucial for neuronal function.
  • The role of tau in muscle tissue, particularly during denervation, is not well understood.
  • Phosphorylated tau (p-tau) is implicated in neurodegenerative diseases.

Purpose of the Study:

  • To investigate the effect of denervation on tau expression and phosphorylation in rat soleus muscles.
  • To elucidate the degradation pathways of tau in denervated muscle.
  • To explore the potential role of tau accumulation in muscular atrophy.

Main Methods:

  • Immunoblot analysis to quantify tau levels.
  • Immunohistochemistry to assess tau and beta-tubulin localization.
  • Treatment with chloroquine to inhibit lysosomal degradation.

Related Experiment Videos

  • Comparison between denervated and contralateral innervated muscles.

    • Main Results:

    • Tau levels, particularly phosphorylated tau, significantly increased in denervated soleus muscles compared to innervated controls.
    • Both tau and beta-tubulin showed increased co-localization in the sarcoplasm of denervated muscles.
    • Chloroquine treatment led to accumulation of phosphorylated tau within autophagic vacuoles in denervated muscle.
    • Evidence suggests tau is degraded via both lysosomal and non-lysosomal (calpain) systems.

    Conclusions:

    • Denervation induces accumulation of phosphorylated tau and beta-tubulin in rat soleus muscle.
    • This accumulation may play a role in preserving muscle fiber integrity during atrophy or regeneration.
    • Tau degradation in muscle involves autophagy, lysosomal, and calpain pathways.