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PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: application to
L A Metherell1, J M Logan, J Stanley
1Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
Journal of Clinical Microbiology
|January 16, 1999
Abstract:
We report a PCR-enzyme-linked immunosorbent assay which identifies Campylobacter species by capture hybridization of a single-stranded 16S rRNA gene amplicon with species-specific probes in a microtiter plate format. Specificities were confirmed for both reference and field strains, but the type strain of Campylobacter coli was atypical. The assay was rapid, informative, and usable with stool-extracted DNA.