Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modified hemoglobin blood substitutes: present status and future perspectives

T M Chang1

  • 1Department of Physiology, McGill University, Montreal, Québec, Canada. artcell@physio.mcgill.ca

Biotechnology Annual Review
|January 16, 1999
PubMed
Summary

First-generation blood substitutes, created using cross-linking and microencapsulation of hemoglobin, offer safe and storable alternatives to red blood cells. Ongoing trials explore their use in surgery and trauma, with advanced versions in development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does conventional early life academic excellence predict later life scientific discovery? An assessment of the lives of great medical innovators.

QJM : monthly journal of the Association of Physicians·2020
Same author

Propentdyopent: the scaffold of a heme metabolite as an electron reservoir in transition metal complexes.

Chemical communications (Cambridge, England)·2016
Same author

Androgen receptor (AR) differential roles in hormone-related tumors including prostate, bladder, kidney, lung, breast and liver.

Oncogene·2013
Same author

Microencapsulation of enzymes, cells, and genetically engineered microorganisms.

Methods in molecular medicine·2011
Same author

Structure and dynamics of N,N-diethyl-N-methylammonium triflate ionic liquid, neat and with water, from molecular dynamics simulations.

The journal of physical chemistry. A·2010
Same author

Neurohormonal control of exocrine pancreas.

Current opinion in gastroenterology·2006

Area of Science:

  • Biotechnology
  • Biomedical Engineering
  • Hematology

Background:

  • Red blood cells (RBCs) are crucial for oxygen transport but face limitations like short shelf-life and transfusion reactions.
  • Current blood transfusion practices require careful blood typing and cross-matching, consuming significant time and resources.
  • Infectious agents like HIV and hepatitis necessitate stringent screening of blood donations.

Purpose of the Study:

  • To introduce novel blood substitutes based on modified hemoglobin.
  • To highlight the advantages of these substitutes over traditional RBC transfusions.
  • To discuss ongoing clinical trials and future directions in blood substitute development.

Main Methods:

  • Biotechnological modification of hemoglobin through cross-linking and microencapsulation.

Related Experiment Videos

  • Sterilization techniques including pasteurization, ultrafiltration, and chemical treatments.
  • Utilizing hemoglobin from various sources such as human, bovine, and recombinant forms.

    • Main Results:

    • Developed blood substitutes are sterilizable, eliminating transfusion-transmitted infections.
    • Absence of blood group antigens negates the need for cross-matching, enabling immediate transfusion.
    • Modified hemoglobins demonstrate extended storage capabilities compared to RBCs.
    • Clinical trials are evaluating efficacy in surgical and trauma settings.

    Conclusions:

    • Cross-linked hemoglobins represent a significant advancement as first-generation blood substitutes.
    • These substitutes offer enhanced safety, convenience, and storage stability.
    • Further research is focused on next-generation substitutes with more comprehensive RBC functions.