Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Zebrafish YAC, BAC, and PAC genomic libraries

C T Amemiya1, T P Zhong, G A Silverman

  • 1Center for Human Genetics, Boston University School of Medicine, Massachusetts 02118-2394, USA.

Methods in Cell Biology
|January 19, 1999
PubMed
Summary

Positional cloning in zebrafish utilizes large-insert genomic resources like yeast, bacterial, and P1-derived artificial chromosomes. These tools facilitate gene discovery for developmental defects, offering advantages over human and mouse studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emergence of Functional Heart-Brain Circuits in a Vertebrate.

bioRxiv : the preprint server for biology·2025
Same author

Resistance to inflammation underlies enhanced fitness in clonal hematopoiesis.

Science (New York, N.Y.)·2021
Same author

Transplantation in zebrafish.

Methods in cell biology·2017
Same author

Chemical screening in zebrafish for novel biological and therapeutic discovery.

Methods in cell biology·2017
Same author

c-myb hyperactivity leads to myeloid and lymphoid malignancies in zebrafish.

Leukemia·2016
Same author

Tissue-specific gene targeting using CRISPR/Cas9.

Methods in cell biology·2016

Area of Science:

  • Developmental Biology
  • Genetics
  • Zebrafish Research

Background:

  • Zebrafish are a key model organism for studying gene function and developmental biology.
  • Numerous developmental defects in zebrafish necessitate the identification of causative genes.
  • Positional cloning is a critical technique for isolating genes underlying genetic disorders.

Purpose of the Study:

  • To review available large-insert genomic resources for zebrafish positional cloning.
  • To discuss the screening and application of these resources in positional cloning strategies.
  • To highlight the logistical considerations and advantages of zebrafish positional cloning.

Main Methods:

  • Review of yeast artificial chromosome (YAC), bacterial artificial chromosome (BAC), and P1-derived artificial chromosome (PAC) libraries.

Related Experiment Videos

  • Discussion of screening methodologies for identifying target clones within these libraries.
  • Analysis of positional cloning strategies and their implementation in zebrafish.

    • Main Results:

    • Availability of YAC, BAC, and PAC large-insert genomic resources for the zebrafish community.
    • Detailed discussion on screening methods and application of these resources in positional cloning.
    • Identification of unique biological advantages that simplify zebrafish positional cloning compared to other model organisms.

    Conclusions:

    • Zebrafish positional cloning projects are facilitated by large-insert genomic resources.
    • Despite logistical challenges, zebrafish offer a comparatively easier system for gene isolation.
    • Advancements in mapping reagents and information will accelerate gene identification and biological studies in zebrafish.