Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The age-associated decrease in the amount of amplifiable full-length mitochondrial DNA in human skeletal muscle

S A Kovalenko1, G Kopsidas, M M Islam

  • 1Centre For Molecular Biology and Medicine, Epworth Medical Centre, Melbourne, Victoria, Australia. SKovalenko@cmbm.com.au

Biochemistry and Molecular Biology International
|January 19, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid rise in COVID-19 among young people in England - learning for the future.

Public health·2023
Same author

Prediction of Coronary Artery Disease Severity by Using CHA2DS2-VASC-HSF Score in Patients with ST-Elevation Myocardial Infarction.

Mymensingh medical journal : MMJ·2023
Same author

Diagnostic Dilemma of Frozen Shoulder in Post CABG Patient: A Case Report.

Mymensingh medical journal : MMJ·2023
Same author

Towards next generation digital twin in robotics: Trends, scopes, challenges, and future.

Heliyon·2023
Same author

Observation of Myocardial Involvement in Patients Recovered From COVID-19 by Using Cardiac Magnetic Resonance Imaging, In a Tertiary Care Hospital, Bangladesh.

Mymensingh medical journal : MMJ·2022
Same author

A Case of Bilateral Middle Cerebral Artery Stenosis with Unilateral M1 Stenting.

Mymensingh medical journal : MMJ·2022
Same journal

Stability of assembled epitopes on human chorionic gonadotropin to covalent modifications.

Biochemistry and molecular biology international·2009
Same journal

Expression or possession of catalase gene does not alter the capacity of M. smegmatis to survive within human macrophages.

Biochemistry and molecular biology international·2009
Same journal

Quantification of epididymal retinoic acid-binding protein (ERABP) mRNA in different anatomical regions of the adult rat epididymis.

Biochemistry and molecular biology international·2009
Same journal

Efficient production of active TNF-alpha by albumin signal peptide.

Biochemistry and molecular biology international·2009
Same journal

The priming effects of some human interleukins on the production of leukotrienes by calcium ionophore stimulated leukocytes.

Biochemistry and molecular biology international·2009
Same journal

Age- and Tissue-specific regulation of chicken inorganic pyrophosphatase.

Biochemistry and molecular biology international·2009
See all related articles

Mitochondrial DNA (mtDNA) mutations increase with age, reducing energy production. Functional, amplifiable mtDNA declines significantly in aging muscle, despite total mtDNA levels remaining stable.

Area of Science:

  • Cellular and Molecular Biology
  • Aging Research
  • Mitochondrial Biology

Background:

  • Mitochondrial DNA (mtDNA) mutations are linked to aging and decreased cellular energy production.
  • The precise impact of age-related mtDNA alterations on functional capacity requires further elucidation.

Purpose of the Study:

  • To investigate the age-related changes in full-length mtDNA (FLmtDNA) and its correlation with mitochondrial bioenergy.
  • To determine if total mtDNA content or the integrity of mtDNA changes with age in human muscle.

Main Methods:

  • Analysis of single human quadriceps muscle fibers.
  • Extra-long PCR (XL-PCR) to quantify amplifiable FLmtDNA.
  • Measurement of cytochrome c oxidase activity as a marker of mitochondrial bioenergy.

Related Experiment Videos

  • Southern blotting of unamplified genomic DNA to assess total FLmtDNA.

    • Main Results:

    • A wide range of mtDNA deletion mutations accumulate with age.
    • The amount of amplifiable FLmtDNA significantly decreases with age in human quadriceps muscle.
    • A strong correlation exists between reduced amplifiable FLmtDNA and diminished cytochrome c oxidase activity.
    • Southern blotting indicates minimal change in total FLmtDNA content in aged muscle.

    Conclusions:

    • Age-related mtDNA damage, rather than a decrease in total mtDNA quantity, leads to reduced functional mtDNA.
    • The decline in amplifiable FLmtDNA with age correlates with impaired mitochondrial bioenergetics.
    • This suggests that accumulated mtDNA damage is a key factor in age-associated decline of mitochondrial function.