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Quantitative determination of domperidone in rat plasma by high-performance liquid chromatography with fluorescence
K Yamamoto1, M Hagino, H Kotaki
1Department of Pharmacy, The University of Tokyo Hospital, Faculty of Medicine, The University of Tokyo, Japan.
Journal of Chromatography. B, Biomedical Sciences and Applications
|January 19, 1999
Abstract:
A sensitive and selective analytical method for the determination of domperidone in rat plasma is described. The procedure involves liquid-liquid extraction followed by reversed-phase high-performance chromatographic analysis with fluorometric detection at 282 nm for excitation and 328 nm for emission. The detection limit was 1 ng ml(-1) using 1 ml of plasma. This assay procedure should be useful for the pharmacokinetic study of domperidone in small animals such as rats.