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[Repeat structure of DYS389 locus]

T Komuro1, H Tsutsumi, R Mukoyama

  • 1Department of Legal Medicine, Nihon University School of Dentistry, Tokyo, Japan.

Nihon Hoigaku Zasshi = the Japanese Journal of Legal Medicine
|January 20, 1999
PubMed
Summary

The DYS389 locus exhibits complex tetranucleotide repeats, leading to polymorphic amplification products DYS389 I and DYS389 II. These variations are valuable for individual identification and paternity testing.

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Area of Science:

  • Forensic Genetics
  • Human Molecular Genetics
  • Population Genetics

Context:

  • The DYS389 locus contains complex, polymorphic tetranucleotide repeat units (CTGT and CTAT).
  • Primer design for DYS389 results in two distinct amplification products, DYS389 I and DYS389 II, both displaying polymorphism.
  • Polymorphisms arise from variations in the number of CTGT and CTAT repeats within these regions.

Purpose:

  • To analyze the length polymorphisms and repeat structures of the DYS389 locus.
  • To investigate the utility of DYS389 locus variations for forensic applications.
  • To characterize the allelic structures of DYS389 I and DYS389 II.

Summary:

  • DNA from 68 males (dental pulp) and 48 males (saliva) was analyzed for DYS389 locus polymorphisms using denaturing polyacrylamide gel electrophoresis and sequencing.
  • DYS389 I alleles ranged from 8-12 repeats (r), with allele 11 being most frequent. DYS389 II alleles ranged from 24-29 repeats (p+q+r), with allele 27 most frequent.
  • Allele structures were confirmed as (CTGT)3(CTAT)r for DYS389 I and (CTGT)p(CTAT)q(CTGT)3(CTAT)r for DYS389 II, with specific repeat number ranges identified. Notably, DYS389 II alleles of the same size exhibited different repeat structures.

Impact:

  • The DYS389 locus demonstrates significant potential for individual identification due to its complex and variable repeat structures.
  • The characterized polymorphisms and repeat variations at the DYS389 locus enhance its effectiveness in paternity testing.
  • Understanding the intricate repeat structures of DYS389 contributes to advancements in forensic DNA analysis and human genetic research.

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