[The liquid scintillation counting of the 14C natural level in ethanol: a comparison of different standardisation methods (author's transl)]
Related Experiment Video
Updated: Oct 1, 2026

Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography
Published on: September 5, 2012
Abstract:
The influence on liquid scintillation counting efficiency of internal standards toluene-14C, hexadecane-14C and ethanol-14C has been studied for low level-14C measuring of ethanol samples. Many biological and synthetic samples of ethanol have been counted: the best results are obtained with external standardisation on quenching calibration curve made with ethanol-14C. External standardisation on quenching calibration curve made with toluene-14C is preferable to internal standardisation with ethanol-14C. Hesadecane-14C as internal standard gives activities rather higher than true values.
Related Concept Videos
Chemical Shift: Internal References and Solvent Effects
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
Atomic Absorption Spectroscopy: Lab
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

