Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new oxisuran metabolite

F J Leinweber, R C Greenough, F J Di Carlo

    Xenobiotica; the Fate of Foreign Compounds in Biological Systems
    |October 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Researchers biosynthesized an unidentified oxisuran metabolite in rat liver cytosol, identifying it as oxisuran alcohol sulphide. This study elucidates oxisuran metabolism pathways and enzymatic transformations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Thermal decomposition of thonzonium bromide.

    Pharmaceutical research·2013
    Same author

    alpha-Amylase from Bacillus subtilis; purification and physical properties.

    Archives of biochemistry·2010
    Same author

    alpha-Amylase from Bacillus subtilis; essential groups.

    Archives of biochemistry·2010
    Same author

    Human cytochrome P450 enzymes: a status report summarizing their reactions, substrates, inducers, and inhibitors.

    Drug metabolism reviews·1997
    Same author

    Assessment of carcinogenic hazard of chemical mixtures through analysis of binary chemical interaction data.

    Environmental health perspectives·1994
    Same author

    Ranking the carcinogenic potential of chemical mixtures: the integral search system and its use in evaluating hazardous waste sites.

    Drug metabolism reviews·1994
    Same journal

    Evaluation of the effects of paeoniflorin on cytochrome P450 isozymes in rats.

    Xenobiotica; the fate of foreign compounds in biological systems·2026
    Same journal

    Berberine Hydrochloride Induces Apoptosis in Triple-Negative Breast Cancer Cells Through Modulation of the sirtuin/p65 Signaling Axis.

    Xenobiotica; the fate of foreign compounds in biological systems·2026
    Same journal

    Computational analysis of dual targeting of β<sub>1</sub>-adrenergic and AT<sub>1</sub> receptors by bisoprolol fumarate and candesartan cilexetil for hypertensive modulation.

    Xenobiotica; the fate of foreign compounds in biological systems·2026
    Same journal

    Total saponins from ginseng stems and leaves antagonise acute lead-cadmium co-exposure-induced oxidative liver damage in mice via the Keap1/Nrf2 signalling pathway.

    Xenobiotica; the fate of foreign compounds in biological systems·2026
    Same journal

    Intestinal deglycosylation activates saikosaponins as potent, selective UGT2B7/2B15 inhibitors: structural basis and implications for herb-drug interactions.

    Xenobiotica; the fate of foreign compounds in biological systems·2026
    Same journal

    Tetrahydrouridine pre-treatment increases tissue exposure of <sup>14</sup>C-decitabine in mice.

    Xenobiotica; the fate of foreign compounds in biological systems·2026
    See all related articles

    Area of Science:

    • Pharmacology and Toxicology
    • Biochemistry
    • Metabolism Studies

    Background:

    • Oxisuran is a xenobiotic compound with observed metabolites in animal urine.
    • Understanding the metabolic fate of oxisuran is crucial for assessing its biological impact.

    Purpose of the Study:

    • To biosynthesize and identify an unknown oxisuran metabolite found in animal urine.
    • To elucidate the enzymatic pathways involved in oxisuran metabolism using rat liver fractions.

    Main Methods:

    • Incubation of [14C]oxisuran with rat liver cytosol for biosynthesis.
    • Isolation and identification of the metabolite using preparative thin-layer chromatography (t.l.c.) and mass fragmentography.
    • Confirmation of identity through biosynthesis from oxisuran sulphide.

    Related Experiment Videos

    Main Results:

    • An unidentified oxisuran metabolite was successfully biosynthesized and identified as oxisuran alcohol sulphide.
    • Rat liver cytosol was more effective than the 9000 g supernatant in reducing oxisuran to its alcohol sulphide.
    • Neither rat liver fraction could reduce oxisuran alcohol sulphoxides to sulphide, but the 9000 g fraction oxidized oxisuran and its sulphoxide to oxisuran alcohol sulphone.

    Conclusions:

    • Oxisuran alcohol sulphide is a primary metabolite of oxisuran formed via reduction in rat liver cytosol.
    • Specific enzymatic activities in rat liver fractions dictate the reductive and oxidative pathways of oxisuran metabolism.