Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ligand-induced structural changes in amylose partially complexed with iodine

S V Bhide, N R Kale

    Biochimica Et Biophysica Acta
    |October 22, 1976
    PubMed
    Summary

    Complexing agents alter the blue amylose-iodine complex formation. Higher agent concentrations and temperature changes induce structural shifts in amylose, affecting its spectral properties.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Marginal discrepancy as affected by selective placement of die-spacer: an in vitro study.

    Journal of Indian Prosthodontic Society·2013
    Same author

    Accuracy of ringless casting and accelerated wax-elimination technique: a comparative in vitro study.

    Journal of prosthodontics : official journal of the American College of Prosthodontists·2013
    Same author

    Assessment of the impact strength of the denture base resin polymerized by various processing techniques.

    Indian journal of dental research : official publication of Indian Society for Dental Research·2013
    Same author

    Efficacy of different disinfectant systems on alginate and addition silicone impression materials of Indian and international origin: a comparative evaluation.

    Journal of Indian Prosthodontic Society·2011
    Same author

    Influence of the processing technique on the flexural fatigue strength of denture base resins: an in vitro investigation.

    Indian journal of dental research : official publication of Indian Society for Dental Research·2010
    Same author

    An isolectin complex from Trichosanthes anguina seeds.

    Phytochemistry·2000

    Area of Science:

    • Biochemistry
    • Polymer Chemistry

    Background:

    • The amylose-iodine complex is a well-known system used to study starch structure.
    • Understanding factors influencing complex formation is crucial for applications in food science and materials.

    Purpose of the Study:

    • To investigate the effect of various complexing agents (alcohols) on the formation and spectral properties of the amylose-iodine complex.
    • To explore the structural changes in amylose induced by these agents under specific conditions.

    Main Methods:

    • Spectrophotometric analysis (absorbance at 640 nm) was employed.
    • The study examined the influence of different alcohol concentrations and temperature variations.
    • Complex formation was studied under suboptimum iodine concentrations and without potassium iodide.

    Main Results:

    • Complexing agents, particularly at higher concentrations, caused a decrease in absorbance at 640 nm and a blue shift in the spectrum.
    • Temperature elevation led to a reversible decrease in absorbance, suggesting a helix-coil transition.
    • Ligand-induced structural changes in the amylose chain were proposed as the mechanism.

    Conclusions:

    • Complexing agents significantly modulate the amylose-iodine complex formation and stability.
    • Amylose undergoes temperature-dependent helix-to-random coil transitions in aqueous systems influenced by complexing agents.
    • These findings provide insights into the molecular interactions governing amylose structure and complexation.

    Related Experiment Videos