Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Messages from ultrahigh resolution crystal structures

S Longhi1, M Czjzek, C Cambillau

  • 1Laboratoire d'Architecture et Fonction de Macromolécules Biologiques (AFMB) CNRS-UPR9039, IBSM 31 Chemin Joseph-Aiguier 13402 Marseille cedex 20 France.

Current Opinion in Structural Biology
|January 23, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiovascular reflex tests detect autonomic dysfunction in symptomatic and pre-symptomatic subjects with hereditary transthyretin amyloidosis.

Clinical autonomic research : official journal of the Clinical Autonomic Research Society·2023
Same author

Correction to: A non-classical presentation of APECED in a family with heterozygous R203X AIRE gene mutation.

Journal of endocrinological investigation·2022
Same author

A non-classical presentation of APECED in a family with heterozygous R203X AIRE gene mutation.

Journal of endocrinological investigation·2022
Same author

[Structural disorder within the replicative complex of measles virus: functional implications].

Virologie (Montrouge, France)·2021
Same author

Non-Bloch-Band Collapse and Chiral Zener Tunneling.

Physical review letters·2020
Same author

Probing the dynamic properties of two sites simultaneously in a protein-protein interaction process: a SDSL-EPR study.

Physical chemistry chemical physics : PCCP·2019
Same journal

Molecular crowding and amyloidogenic self-assembly: Emergent perspectives from modern computations.

Current opinion in structural biology·2026
Same journal

Recent advances in modeling and simulation of biological phenomena in crowded and cellular environments.

Current opinion in structural biology·2026
Same journal

Progress toward linking single-molecule behavior and condensate material properties.

Current opinion in structural biology·2026
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
See all related articles

High-resolution macromolecular structures provide unbiased data on protein stereochemistry and refinement parameters. This enables detailed analysis of protein geometry, active sites, and interactions, advancing structural biology.

Area of Science:

  • Structural Biology
  • Biochemistry
  • Computational Chemistry

Background:

  • The increasing number of macromolecular crystal structures determined at atomic resolution provides a robust dataset.
  • This dataset allows for statistically unbiased analysis of protein stereochemistry and refinement parameter validity.

Purpose of the Study:

  • To leverage the growing repository of high-resolution structures for detailed analysis.
  • To validate computational methods and understand molecular interactions through structural data.

Main Methods:

  • Analysis of a large dataset of atomic-resolution macromolecular crystal structures.
  • Utilizing structural data to assess protein stereochemistry and refinement parameters.
  • Applying structural insights to validate normal mode calculations and analyze charge density distributions.

Related Experiment Videos

Main Results:

  • Statistically unbiased data on protein stereochemistry and refinement parameters are now obtainable.
  • High-resolution structures facilitate precise determination of protein and active site geometry.
  • Analysis of hydration shells and interactions within protein complexes is enhanced.

Conclusions:

  • The critical size of structural data enables reliable validation of protein models and computational methods.
  • High-resolution structures are crucial for understanding protein-ligand and protein-prosthetic group interactions.
  • This structural information is vital for deriving physical parameters of molecular interactions.