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Updated: Aug 17, 2026

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Published on: March 9, 2012
Regulation of tubulin polypeptides and microtubule function: Luv1p [correction of Rki1p] interacts with the
A M Smith1, J E Archer, F Solomon
1Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Abstract:
Genetic analysis of microtubule functions in the yeast Saccharomyces cerevisiae suggests that cells manage the levels and activities of the tubulin polypeptides. These reactions may be involved in protein folding, formation of the heterodimer, and maintenance of the appropriate balance between alpha- and beta-tubulin. One protein involved in these functions is Rbl2p, which forms a complex with beta-tubulin. Here we describe the identification of a novel yeast gene, LUV1 [corrected], that interacts genetically with RBL2. Deletion of rki1 causes conditional defects in microtubule assembly and cell growth. Luv1p [corrected] can be isolated in a complex containing Rbl2p. The results support the existence of cellular mechanisms for regulating microtubule function through the tubulin polypeptides.
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