Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluoxetine action upon human T lymphocyte proliferation

V Ayelli Edgar1, A M Genaro, G A Cremaschi

  • 1Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnológicas (CONICET), Buenos Aires, Argentina.

Acta Physiologica, Pharmacologica Et Therapeutica Latinoamericana : Organo De La Asociacion Latinoamericana De Ciencias Fisiologicas Y [De] La Asociacion Latinoamericana De Farmacologia
|January 23, 1999
PubMed
Summary

Fluoxetine shows a dual effect on human T lymphocyte proliferation, inhibiting it at high activation levels while stimulating it at lower levels. This modulation involves protein kinase C (PKC) and cyclic adenosine monophosphate (cAMP) pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to: The thyroid status reprograms T cell lymphoma growth and modulates immune cell frequencies.

Journal of molecular medicine (Berlin, Germany)·2022
Same author

Participation of hypothalamic CB1 receptors in reproductive axis disruption during immune challenge.

Journal of neuroendocrinology·2017
Same author

Alteration of neurotrophin and cytokine expression in lymphocytes as novel peripheral markers of spatial memory deficits induced by prenatal stress.

Physiology & behavior·2017
Same author

Selective cytoprotective effect of histamine on doxorubicin-induced hepatic and cardiac toxicity in animal models.

Cell death discovery·2016
Same author

Histamine: a potential cytoprotective agent to improve cancer therapy?

Cell death & disease·2016
Same author

The thyroid status reprograms T cell lymphoma growth and modulates immune cell frequencies.

Journal of molecular medicine (Berlin, Germany)·2015

Area of Science:

  • Immunology
  • Pharmacology
  • Cell Biology

Background:

  • T lymphocytes play a crucial role in immune responses.
  • Fluoxetine is a selective serotonin reuptake inhibitor with potential immunomodulatory effects.
  • Understanding drug effects on immune cells is vital for therapeutic applications.

Purpose of the Study:

  • To investigate the impact of fluoxetine on human T lymphocyte proliferation.
  • To analyze early signaling events, including cyclic adenosine monophosphate (cAMP) formation, following T cell activation.
  • To assess the role of Protein Kinase C (PKC) in fluoxetine's immunomodulatory effects.

Main Methods:

  • Human T lymphocytes were isolated from normal volunteers.
  • Cells were cultured with or without Concanavalin A (Con A) and fluoxetine.

Related Experiment Videos

  • Measurements included cellular proliferation, Protein Kinase C (PKC) levels, and cyclic adenosine monophosphate (cAMP) formation.

    • Main Results:

    • Fluoxetine inhibited T lymphocyte proliferation at mitogenic Con A concentrations (2 µg/ml), associated with PKC degradation and increased cAMP.
    • Fluoxetine stimulated T lymphocyte response and increased PKC translocation at submitogenic Con A concentrations (1 µg/ml).
    • Extracellular calcium mobilization and subsequent modulation of calcium influx appear to be involved in these effects.

    Conclusions:

    • Fluoxetine exhibits dose-dependent immunomodulatory effects on human T lymphocytes.
    • The mechanisms involve modulation of PKC translocation and cAMP formation, potentially influenced by calcium influx.
    • Fluoxetine may modulate the immune response through intricate signaling pathways involving T cells.