Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Search for New Physics in Jet Multiplicity Patterns of Multilepton Events at sqrt[s]=13 TeV.

Physical review letters·2025

Same author

Prevalence and antimicrobial susceptibility profile of uropathogens in dogs and cats with signs of urinary tract infection.

The Journal of small animal practice·2024

Same author

Visual cues added to a virtual environment paradigm do not improve motor arrests in Parkinson's disease.

Journal of neural engineering·2021

Same author

Validation of the Auditory Stroop Task to increase cognitive load in walking tasks in healthy elderly and persons with Parkinson's disease.

PloS one·2019

Same author

Neuroendocrine Tumor of the Pancreas as a Manifestation of Cowden Syndrome: A Case Report.

The Journal of clinical endocrinology and metabolism·2015

Same author

Blood flow velocity measured using the Retinal Function Imager predicts successful ranibizumab treatment in neovascular age-related macular degeneration: early prospective cohort study.

Eye (London, England)·2015

Search research articles

Related Experiment Video

Updated: Jun 24, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 4, 2010

Expression profiling using cDNA microarrays

D J Duggan¹, M Bittner, Y Chen

¹Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Nature Genetics

|January 23, 1999

Summary

This review covers cDNA microarray technology for gene expression profiling. It details array fabrication, target labeling, and data analysis for thousands of genes.

More Related Videos

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Related Experiment Videos

Last Updated: Jun 24, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 4, 2010

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

cDNA microarrays enable high-throughput gene expression profiling.
They analyze tens of thousands of genes simultaneously.

Purpose of the Study:

To review the technical aspects of cDNA microarray technology.
To cover principles, fabrication, labeling, and data analysis.

Main Methods:

Review of existing literature and technical protocols.
Focus on DNA target arraying, cDNA probing, and fluorescent/radioactive labeling.

Main Results:

Detailed explanation of cDNA microarray principles.
Overview of array fabrication, target labeling techniques.
Discussion of image analysis, data extraction, management, and mining.

Conclusions:

cDNA microarrays are a powerful tool for gene expression analysis.
Technical understanding is crucial for effective application and data interpretation.