Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleolin: a multifunctional major nucleolar phosphoprotein

R Tuteja1, N Tuteja

  • 1International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.

Critical Reviews in Biochemistry and Molecular Biology
|January 26, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasmodium falciparum signal recognition particle components and anti-parasitic effect of ivermectin in blocking nucleo-cytoplasmic shuttling of SRP.

Cell death & disease·2014
Same author

Serum acetylcholinesterase activity in psychiatric patients.

Indian journal of psychiatry·2011
Same author

Isolation and characterization of a phospholipase C delta isoform from pea that is regulated by light in a tissue specific manner.

Molecular genetics and genomics : MGG·2003
Same author

Molecular mechanisms of DNA damage and repair: progress in plants.

Critical reviews in biochemistry and molecular biology·2001
Same author

Unraveling DNA repair in human: molecular mechanisms and consequences of repair defect.

Critical reviews in biochemistry and molecular biology·2001
Same author

A pea homologue of human DNA helicase I is localized within the dense fibrillar component of the nucleolus and stimulated by phosphorylation with CK2 and cdc2 protein kinases.

The Plant journal : for cell and molecular biology·2001
Same journal

Conformational mobility of cationic voltage-gated ion channels.

Critical reviews in biochemistry and molecular biology·2026
Same journal

DNA polymerase I: structure, activity, and function in bacterial DNA replication and repair.

Critical reviews in biochemistry and molecular biology·2026
Same journal

The impact of HIV-1 Nef on CD4<sup>+</sup> T cells.

Critical reviews in biochemistry and molecular biology·2026
Same journal

Recent insights into bacterial ESCRT-III-mediated membrane remodeling.

Critical reviews in biochemistry and molecular biology·2026
Same journal

CRISPR-Cas9 editing of agricultural crops and medicinal plants: toward a cornucopia of natural products.

Critical reviews in biochemistry and molecular biology·2025
Same journal

RNA modifications as a means of self-recognition and immune protection.

Critical reviews in biochemistry and molecular biology·2025
See all related articles

Nucleolin, a vital eukaryotic protein, plays diverse roles in cell growth and metabolism. Its complex structure and multiple functions, including DNA/RNA binding and helicase activity, are crucial for cellular processes.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Nucleolin is a highly conserved, abundant protein in exponentially growing eukaryotic cells, primarily located in the nucleolus.
  • It possesses a unique tripartite structure with distinct domains responsible for interactions with DNA, RNA, and proteins.
  • Nucleolin contains a bipartite nuclear localization signal sequence, indicating its active transport into the nucleus.

Purpose of the Study:

  • To elucidate the multifaceted roles and structural characteristics of nucleolin in eukaryotic cells.
  • To explore the diverse biochemical activities and regulatory mechanisms of nucleolin.
  • To understand nucleolin's involvement in various cellular processes, including ribosome biogenesis and cell proliferation.

Main Methods:

Related Experiment Videos

  • Bioinformatic analysis of nucleolin's conserved structure and localization signals.
  • Biochemical assays to characterize nucleolin's DNA/RNA binding, helicase, and ATPase activities.
  • Literature review and integration of studies on nucleolin's involvement in cellular pathways.

    • Main Results:

    • Nucleolin exhibits intrinsic self-cleaving, DNA helicase, RNA helicase, and DNA-dependent ATPase activities.
    • It functions as a sequence-specific RNA binding protein, an autoantigen, and a component of a B cell transcription factor.
    • Phosphorylation by specific kinases (cdc2, CK2, PKC-zeta) modulates nucleolin's activities.
    • Nucleolin is implicated in ribosome biogenesis, cell cycle regulation, DNA replication, and signal transduction.

    Conclusions:

    • Nucleolin is a multifunctional protein critical for numerous cellular processes, particularly ribosome biogenesis and cell growth.
    • Its complex structure, diverse activities, and post-translational modifications present a challenging yet fascinating area of study.
    • Understanding nucleolin's regulation is key to comprehending fundamental cellular mechanisms in eukaryotes, including plants.