Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fas receptor expression on asthmatic eosinophils

J E Fahy1, S F Quan, J W Bloom

  • 1Department of Medicine, Respiratory Sciences Center, University of Arizona College of Medicine, Tucson 85724, USA.

Research Communications in Molecular Pathology and Pharmacology
|January 27, 1999
PubMed
Summary

Fas receptor expression on eosinophils did not differ between individuals with asthma and healthy individuals. This finding suggests Fas receptor is not a key factor in asthma-related eosinophil regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of antireflux treatment on the frequency of awakenings from sleep in patients with Gastroesophageal reflux disease.

Neurogastroenterology and motility·2014
Same author

The penetration of antibacterial substances into ischemic, inflammatory tissue; with a consideration of their use in shock of bacterial origin.

The American journal of the medical sciences·2010
Same author

Increased oesophageal acid exposure at the beginning of the recumbent period is primarily a recumbent-awake phenomenon.

Alimentary pharmacology & therapeutics·2010
Same author

Riser's reflux--an eye-opening experience.

Neurogastroenterology and motility·2010
Same author

Control of breathing in children with mild sleep apnoea: a 6-year follow-up study.

The European respiratory journal·2009
Same author

Assembly of the prothrombinase complex.

Biophysical journal·2009

Area of Science:

  • Immunology
  • Cell Biology
  • Respiratory Medicine

Background:

  • The Fas receptor (Fas; CD95) mediates apoptosis, a process crucial for regulating cell populations.
  • Eosinophils play a significant role in allergic inflammation, particularly in asthma.
  • Understanding Fas receptor's role in eosinophils could reveal mechanisms of asthma pathogenesis.

Purpose of the Study:

  • To investigate potential differences in Fas receptor expression on peripheral blood eosinophils between asthmatic and non-asthmatic individuals.
  • To explore the implications of Fas receptor expression for eosinophil apoptosis and regulation in asthma.

Main Methods:

  • Isolation of peripheral blood eosinophils using a multi-step procedure: dextran sedimentation, Ficoll-Hypaque separation, and negative selection with anti-CD16 magnetic beads.

Related Experiment Videos

  • Quantification of Fas receptor expression levels and the percentage of Fas-expressing eosinophils via flow cytometry.

    • Main Results:

    • No significant difference was observed in Fas receptor expression levels on eosinophils between asthmatic (n=9) and non-asthmatic (n=14) subjects.
    • The percentage of eosinophils expressing the Fas receptor was also comparable between the two groups.

    Conclusions:

    • Fas receptor expression on peripheral blood eosinophils is similar in individuals with asthma and healthy individuals.
    • These findings suggest that differences in Fas receptor expression on eosinophils are unlikely to be a primary mechanism driving asthma pathophysiology or eosinophil dysregulation in this condition.