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Stimulation side-dependent asymmetrical secretion of poly I:poly C-induced interferon-beta from polarized epithelial
S Okamoto1, K Nakanishi, Y Watanabe
1Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Sakyo-ku, 606-8501, Japan.
Abstract:
Mode of secretion of poly I:poly C-induced IFN was examined using epithelial cell lines in a bicameral culture system. Although the cell lines formed a tight cell sheet and produced IFN-beta following poly I:poly C treatment in spite of its application to the upper or lower compartment, IFN secretion differed between the apical and basolateral cell membranes. When poly I:poly C was applied to the upper compartment, IFN was secreted predominantly from the apical membrane. Inversely, poly I:poly C applied to the lower compartment caused preferential IFN secretion from the basolateral membrane. These results suggest that in epithelial cells poly I:poly C stimulation induces intracellular membrane traffic toward the stimulation side.
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