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Eggshell membrane structure and penetration by Salmonella typhimurium
M E Berrang1, J F Frank, R J Buhr
1United States Department of Agriculture, Agricultural Research Service, Russell Research Center, Athens, Georgia 30604-5677, USA. mberrang@asrr.arsusda.gov
Journal of Food Protection
|January 28, 1999
Summary
Eggshell membrane structure did not correlate with Salmonella Typhimurium penetration. This study examined broiler eggshell membrane integrity and its role in preventing bacterial contamination.
Area of Science:
- Food safety
- Microbiology
- Animal science
Background:
- Eggshell membrane serves as a natural barrier against microbial contamination.
- Understanding its structural properties is crucial for preventing pathogen entry in poultry products.
Purpose of the Study:
- To investigate the relationship between eggshell membrane structure and Salmonella Typhimurium penetration.
- To assess the structural integrity of broiler eggshell membranes over the flock's productive life.
Main Methods:
- Broiler eggshell membranes were collected monthly and tested for Salmonella Typhimurium penetration using a two-chamber apparatus.
- Membrane structure was analyzed using confocal laser scanning microscopy and image analysis software to measure interfiber areas.
- Bacterial penetration was assessed through cultural analysis of samples collected at various time points.
Main Results:
- No significant correlation was found between the measured interfiber areas of the eggshell membrane and the penetration rate of Salmonella Typhimurium.
- Bacterial penetration was detected over a 48-hour period, indicating potential breaches in the membrane barrier.
Conclusions:
- The structural characteristics of the eggshell membrane, specifically interfiber area, do not appear to be a reliable predictor of Salmonella Typhimurium penetration.
- Further research is needed to identify other factors influencing eggshell membrane's barrier function against bacterial pathogens.