Medium-chain-length poly(beta-hydroxyalkanoate) synthesis from triacylglycerols by Pseudomonas saccharophila

Pseudomonas saccharophila NRRL B-628 is capable of utilizing agricultural lipids for growth. The organism exhibited good growth with triacylglycerol substrates that contained saturated fatty acyl moieties such as coconut oil (CO; C10-12 fatty acids) and tallow (T; C16-18 fatty acids). Electron micrographs of the triacylglycerol-grown cells showed the presence of intracellular granules indicative of poly(beta-hydroxyalkanoate) (PHA) production. Cells grown in a 250-ml CO-containing medium produced ca. 0.2 g of medium-chain-length (mcl)-PHA. Gas chromatographic analysis showed that beta-hydroxyoctanoic acid (30%), beta-hydroxydecanoic acid (40%), and beta-hydroxydodecanoic acid (16%) were the major monomer repeat-units of the CO-derived polymer. The estimated mean molecular mass of the CO-derived mcl-PHA as determined by gel permeation chromatography was 13.1 x 10(4) g/mol with a polydispersity of 3.16.