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Updated: Aug 8, 2026

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
T lymphocyte costimulation mediated by reorganization of membrane microdomains
A Viola1, S Schroeder, Y Sakakibara
1Basel Institute for Immunology, Grenzacherstrasse 487, CH 4005 Basel, Switzerland. viola@bii.ch
Abstract:
Although dispensable, costimulation through CD28 facilitates activation of naïve T lymphocytes. CD28 engagement led to the redistribution and clustering of membrane and intracellular kinase-rich raft microdomains at the site of T cell receptor (TCR) engagements. Although not affecting TCR down-regulation, this process led to higher and more stable tyrosine phosphorylation of several substrates and higher consumption of Lck. These results may provide a general mechanism for amplifying receptor signaling by reorganization of membrane microdomains.
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