A histochemical and morphologic study of postobstructive diuresis in the rat

Area of Science:

• Nephrology
• Renal Physiology
• Pathology

Background:

• Postobstructive diuresis is characterized by renal functional abnormalities.
• These abnormalities suggest changes in tubular and glomerular membrane properties.

Purpose of the Study:

• To investigate the morphologic and ultrastructural changes in a rat model of postobstructive diuresis.
• To correlate these structural changes with functional alterations in the kidney.

Main Methods:

• Light and electron microscopy were used to examine 32 postobstructed rat kidneys and 22 controls.
• Horseradish peroxidase and cytochrome c were infused to assess glomerular and tubular permeability.

Main Results:

• Observed abnormalities included dilated tubules, widened intercellular spaces, and thickened glomerular basement membranes.
• Increased permeability was evidenced by tracer substances in intercellular spaces and cell organelles.
• Reduced enzyme activity and altered cell ratios correlated with impaired concentration and acidification.

Conclusions:

• Renal functional abnormalities in postobstructive diuresis are linked to altered glomerular and tubular permeability.
• Changes in metabolic activity and increased renin activity also contribute to the disorder.