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[Design of constructions for the expression of proteolytic antibody light chain]
A V Demin1, A V Kolesnikov, M A Olfer'ev
1Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Genetika
|February 4, 1999
Abstract:
A new system for the expression of a catalytic light chain antibody to the vasoactive intestinal peptide is described. The system made possible the isolation the large amounts of a homogeneous protein without any additional peptide domains. The preparation obtained can be used in further experiments on light chain crystallization and in X-ray-structural analysis of its catalytic center.