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A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
Published on: March 13, 2011
DNA preparation and efficient microsatellite analysis from insect hemolymph
1Institute for Evolutionary Biology and Ecology, University of Bonn, Germany.
Electrophoresis
|February 5, 1999
Abstract:
A simple and time-saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1-5 microL of insect hemolymph in a Chelex 100-suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population structure and phylogenetic reconstruction can be conducted with minimal experimental influence.

