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Related Experiment Videos

Tissue factor in cultured cells: pharmacologic effects

J R Maynard, D J Fintel, F A Pitlick

    Laboratory Investigation; a Journal of Technical Methods and Pathology
    |December 1, 1976
    PubMed
    Summary

    Pharmacologic agents modulating cell membrane function impact tissue factor activity in WISH amnion cells. Microtubule disruption stabilizes tissue factor, while lysosome stabilizers and transport inhibitors show unclear roles.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Pharmacology

    Background:

    • Tissue factor initiates the coagulation cascade.
    • Regulation of tissue factor activity in cultured cells is not fully understood.
    • WISH amnion cells provide a model for studying tissue factor modulation.

    Purpose of the Study:

    • To investigate the effects of various pharmacologic agents on tissue factor activity in WISH amnion cells.
    • To determine the role of membrane structure, function, and cellular processes in regulating tissue factor.
    • To elucidate the mechanisms underlying tissue factor expression and decay.

    Main Methods:

    • Culturing WISH amnion cells in suspension.
    • Administering pharmacologic agents affecting membrane structure and function (e.g., steroids, chloroquine, DMSO, ouabain, cytochalasin B, colchicine, vinblastine).

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  • Measuring tissue factor activity over time after cell subculture.

    • Main Results:

    • Lysosomal stabilizing steroids suppressed activity changes post-subculture; lytic steroids were ineffective.
    • Chloroquine increased specific activity and prolonged its duration.
    • Microtubule-disrupting agents (colchicine, vinblastine) significantly increased and prolonged tissue factor activity, suggesting stabilization.
    • Agents affecting lysosomes or transport yielded contradictory results.

    Conclusions:

    • Increased membrane fluidity and disrupted secretory processes via microtubule disruption appear to stabilize tissue factor in cultured cells.
    • The roles of lysosomal stability and cellular transport in tissue factor production or decay remain unclear.
    • Pharmacologic modulation of cellular processes offers insights into tissue factor regulation.