Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Clp protease complexes and their diversity in chloroplasts

A Sokolenko1, S Lerbs-Mache, L Altschmied

  • 1Botanisches Institut, Ludwig-Maximilians-Universität, München, Germany.

Planta
|February 10, 1999
PubMed
Summary

Plant chloroplasts contain Clp proteases, ancient ATP-dependent enzymes. This study shows nuclear-encoded ClpP (nClpP) is imported and processed within chloroplasts, forming large protein complexes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

; THE ADVANCEMENTS IN TREATMENT OF HIV-INFECTED PATIENTS WITH HERPETIC INFECTION.

Georgian medical news·2020
Same author

Plant regeneration from leaf protoplasts of evening primrose (Oenothera hookeri).

Plant cell reports·2019
Same author

Tissue culture of wild-type, interspecific genome/plastome hybrids and plastome mutants of evening primrose (Oenothera): controlled morphogenesis and transformation.

Plant cell reports·2019
Same author

Parthenogenetic egg cells of wheat: cellular and molecular studies.

Sexual plant reproduction·2014
Same author

Multiple amounts of DNA related to the size of chloroplasts : I. An autoradiographic study.

Planta·2014
Same author

Genes and transcripts for the P700 chlorophylla apoprotein and subunit 2 of the photosystem I reaction center complex from spinach thylakoid membranes.

Plant molecular biology·2013

Area of Science:

  • Plant molecular biology
  • Protease biochemistry
  • Chloroplast biogenesis

Background:

  • Clp proteases are ancient, ATP-dependent enzymes found in chloroplasts of higher plants.
  • The chloroplast Clp protease complex is composed of nuclear-encoded ClpC (ATPase) and plastid-encoded ClpP (serine protease).
  • A nuclear-encoded ClpP (nClpP) subunit has been identified in tomato.

Purpose of the Study:

  • To investigate the import, processing, and complex formation of nuclear-encoded ClpP (nClpP) in chloroplasts.
  • To characterize the supramolecular complexes formed by ClpC, ClpP, and nClpP.

Main Methods:

  • Import of radiolabeled precursor nClpP into isolated spinach chloroplasts.
  • Analysis of protein processing using gel electrophoresis.

Related Experiment Videos

  • Size-exclusion chromatography and non-denaturing gel electrophoresis to determine complex molecular masses.

    • Main Results:

    • Nuclear-encoded ClpP (nClpP) is synthesized as a precursor and imported into chloroplasts.
    • nClpP undergoes stepwise processing within the chloroplast to its mature form.
    • Distinct heteromeric supramolecular complexes of ClpC, ClpP, and nClpP were identified, with masses ranging from 600 to 1700 kDa.
    • Individual components also formed complexes ranging from 160 to 560 kDa.

    Conclusions:

    • The nuclear-encoded ClpP subunit is integrated into the chloroplast Clp protease system through import and processing.
    • These findings reveal the complex assembly of chloroplast Clp protease machinery, involving both nuclear and plastid-encoded subunits.
    • The formation of large heteromeric complexes highlights the intricate regulation of protease activity within the chloroplast.