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Identification of a structural gene encoding a metallothionein-like domain that includes a putative regulator protein
1Department of Biological Science and Technology, Science University of Tokyo, Chiba, Japan. staguchi@rs.noda.sut.ac.jp
Bioscience, Biotechnology, and Biochemistry
|February 11, 1999
Abstract:
An open reading frame (termed ORF-PR) encoding a metallothionein-like domain-including protein was found upstream of a previously identified Streptomyces chymotrypsin-type protease gene (sam-P20). Promoter and terminator activities of ORF-PR were detected using the promoterless Streptomyces tyrosinase gene as a reporter gene and expression of ORF-PR was supposed to occur before that of sam-P20 gene. Frameshift mutation analysis showed that the ORF-PR product might act as a repressive regulator of the sam-P20 gene.