Related Experiment Videos
Ultrastructural localization of tartrate-resistant acid phosphatase activity in rat osteoblasts
1Department of Anatomy, Okayama University School of Dentistry, Japan. ytoshio@dent.okayama-u.ac.jp
Abstract:
Ultrastructural localization of tartrate-resistant acid phosphatase (TRAP) activity in metaphyseal osteoblasts of young rats was detected and compared with the localization of tartrate-sensitive acid phosphatase (TSAP). TRAP activity was detected in lysosomes and Golgi complex, such as Golgi lamellae, vacuoles and vesicles. TSAP, detected as beta-glycerophosphatase, was also located in Golgi lamellae, vacuoles and vesicles, and in lysosomes. Based on the ultrastructural features of TRAP-positive structures and the similarity of the distribution patterns of TRAP and TSAP, it is suggested that TRAP co-exists with TSAP, and is located in lysosomes and secretory granules in osteoblasts.