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Updated: Aug 15, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
Manganese citrate improves base-calling accuracy in DNA sequencing reactions using rhodamine-based fluorescent
1University of Colorado Cancer Center DNA Sequencing and Analysis Core and Division of Medical Oncology,School of Medicine, University of Colorado Health Sciences Center, Denver,CO 80262, USA. christopher.korch@uchsc.edu
Abstract:
While dideoxy-terminators labeled with rhodamine-based fluorescent dyes provide the most versatile method of automated DNA sequencing, variation in peak heights reduces base-calling accuracy. We describe a simple approach that uses additions of a manganese salt and the metal buffer sodium citrate (MnCit) to overcome this limitation. This modification reduces peak height variability >2-fold and significantly increases the number of accurately read bases in DNA sequences.

