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Updated: Aug 18, 2026
Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
Published on: April 3, 2013
Changes in labeling of soluble and solubilized hippocampus proteins after a learning experiment in rats
Abstract:
At various intervals after acquisition of a brightness discrimination in rats labeled leucine was intraventricularly applied. Hippocampus tissue was fractionated in soluble and solubilized insoluble protein fractions. Protein content and labeling of several electrophoretically resolved bands showed a biphasic time course: a first increase was observed 20 minutes after training including preferably soluble proteins, whereas a second increase (about eight hours after training) was mainly related to solubilized insoluble proteins.
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