C1R levels in normal human sera determined by electroimmunoassay

Area of Science:

• Biochemistry
• Immunology
• Complement System

Background:

• The complement system is crucial for innate and adaptive immunity.
• Understanding the quantitative levels of complement components like C1r is essential for immunological studies.
• Accurate measurement of C1r is necessary for diagnostic and research purposes.

Purpose of the Study:

• To establish reference ranges for C1r levels in normal adult sera.
• To assess the correlation between C1r and other early complement components (C1q, C1s).
• To evaluate the stability of C1r in different sample types and storage conditions.

Main Methods:

• Electroimmunoassay was employed to quantify C1r levels.
• Correlation analysis was performed between C1r, C1q, and C1s.
• C1r stability was tested in serum under various storage conditions (room temperature, freeze-thaw cycles) and in EDTA plasma.

Main Results:

• The 95% reference range for C1r in normal adults was determined to be 71-133% of a normal reference pool.
• C1r levels showed significant positive correlations with C1q (r = 0.708) and C1s (r = 0.768).
• C1r in serum demonstrated stability at room temperature and after repeated freeze-thaw cycles, whereas C1r antigen in EDTA plasma was found to be labile.

Conclusions:

• Electroimmunoassay provides a reliable method for determining C1r levels.
• Established reference ranges and correlations aid in the interpretation of C1r measurements.
• The differential stability of C1r in serum versus EDTA plasma highlights the importance of appropriate sample handling for complement analysis.