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Related Experiment Videos

Toward a dog radiation hybrid map

F Vignaux1, C Priat, S Jouquand

  • 1UPR 41 CNRS Recombinaisons Génétiques, Faculté de Médecine, Rennes, France.

The Journal of Heredity
|February 13, 1999
PubMed
Summary
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This study developed a canine genome map using hybrid cell lines and PCR. These tools aid in canine genetic research and understanding mammalian genome conservation.

Area of Science:

  • Genetics
  • Genomics
  • Comparative Genomics

Background:

  • Developing a comprehensive canine genome map is crucial for genetic research and understanding mammalian genome evolution.
  • Efficiently identifying and characterizing canine genetic markers is essential for linkage studies and comparative genomics.

Purpose of the Study:

  • To create a panel of canine-hamster hybrid cell lines for canine genome mapping.
  • To identify and optimize PCR conditions for dog-specific microsatellites in the presence of hamster DNA.
  • To characterize a set of canine genetic markers, including genes and expressed sequence tags, for future mapping.

Main Methods:

  • Fusion of dog fibroblasts with thymidine kinase-deficient hamster cells to create hybrid cell lines.
  • Polymerase Chain Reaction (PCR) amplification using dog-specific primers for screening and marker analysis.

Related Experiment Videos

  • Microsatellite screening and characterization, including optimization of PCR conditions.
  • Characterization of canine genes, human expressed sequence tags (huESTs), and traced orthologous amplified sequenced tags (TOASTs).

    • Main Results:

    • 107 hybrid cell lines were established and analyzed for the distribution of 39 markers.
    • PCR conditions were optimized for over 500 microsatellites, enabling dog-specific amplification.
    • 254 markers, including canine genes and ESTs, were characterized for mapping potential.
    • The developed hybrid cell lines and markers are valuable resources for canine genome mapping and comparative studies.

    Conclusions:

    • The panel of canine-hamster hybrid cell lines is a valuable resource for constructing a canine genome map.
    • Optimized microsatellites and characterized markers will facilitate further canine linkage studies.
    • These resources will aid in detecting conserved synteny regions across mammalian genomes, including humans.