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Related Experiment Videos

T cell affinity maturation by selective expansion during infection

D H Busch1, E G Pamer

  • 1Sections of Infectious Diseases and Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06511, USA.

The Journal of Experimental Medicine
|February 17, 1999
PubMed
Summary

T cell receptor (TCR) affinity for antigen-MHC complexes increases with T cell expansion during bacterial infection. This selective expansion of high-affinity T cells enhances early detection of infected cells.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Infectious Disease

Background:

  • T lymphocyte recognition of infected cells relies on T cell receptors (TCRs) binding to peptide-MHC complexes.
  • The relationship between TCR affinity and in vivo T cell expansion dynamics remains unclear.

Purpose of the Study:

  • To investigate the impact of TCR affinity on the in vivo expansion of T cell populations following bacterial infection.
  • To determine if higher affinity TCRs are selectively expanded in vivo.

Main Methods:

  • Analysis of T cell populations after bacterial infection in vivo.
  • Assessment of TCR repertoire diversity and affinity for peptide-MHC complexes.
  • Quantification of antigen sensitivity in cytotoxic T lymphocyte assays.

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Main Results:

  • In vivo T cell expansion correlated with an increase in overall TCR affinity for antigen.
  • Expanded T cell populations exhibited a narrower TCR range and higher affinity binding to MHC/peptide complexes.
  • Increased antigen sensitivity was observed in cytotoxic T lymphocyte assays.

Conclusions:

  • Selective expansion of higher-affinity T cells occurs in vivo during bacterial infection.
  • This selection mechanism optimizes the early detection of infected cells by enhancing T cell sensitivity.