Elution of filtered granulocytes for transfusion

Functional and clinical transfusion studies were carried out on granulocytes eluted from nylon filters using ACD Saline solutions and 20% CPD Plasma ACD Saline solutions. Although the average volume of blood and total number of granulocytes filtered were the same, the yield of granulocytes eluted form the filters was 20% greater when 20% CPD plasma was in the eluting solution. Function studies indicated that while the antibacterial capacity of the saline eluted granulocytes was equivalent to that of plasma eluted cells, chemotactic activity was markedly reduced after 24 hours storage in the saline eluted cells. Clinical studies post transfusion also suggest better response from plasma eluted granulocytes.