Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The benzidine technique for histochemical detection of the SO4 ion

W A Hadler, M A da Cruz-Höfling, L M Ziti

    Acta Histochemica
    |January 1, 1978
    PubMed
    Summary

    This study developed a new benzidine histochemical technique for specifically detecting sulfate (SO4) ions in tissues. The improved method prevents interference from phosphate (PO4) ions, enhancing detection accuracy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The effects of low laser irradiation on angiogenesis in injured rat tibiae.

    Histology and histopathology·2004
    Same author

    Neutralization of the pharmacological effects of bothropstoxin-I from Bothrops jararacussu (jararacuçu) venom by crotoxin antiserum and heparin.

    Toxicon : official journal of the International Society on Toxinology·2001
    Same author

    Neutralizing capacity of commercial bothropic antivenom against Bothrops jararacussu venom and bothropstoxin-I.

    Muscle & nerve·2000
    Same author

    Electrophysiological and ultrastructural analysis of the neuromuscular blockade and miotoxicity induced by the Micrurus nigrocinctus snake venom.

    Acta physiologica, pharmacologica et therapeutica latinoamericana : organo de la Asociacion Latinoamericana de Ciencias Fisiologicas y [de] la Asociacion Latinoamericana de Farmacologia·2000
    Same author

    Toxin 2 (PhTx2), a neurotoxic fraction from Phoneutria nigriventer spider venom, causes acute morphological changes in mouse skeletal muscle.

    Toxicon : official journal of the International Society on Toxinology·2000
    Same author

    Bothrops lanceolatus (Fer de lance) venom induces oedema formation and increases vascular permeability in the mouse hind paw.

    Toxicon : official journal of the International Society on Toxinology·2000

    Area of Science:

    • Histochemistry
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Histochemical detection of sulfate (SO4) ions is crucial in biological and medical research.
    • Existing benzidine-based methods for SO4 detection can suffer from interference, particularly from phosphate (PO4) ions.
    • Improving the specificity of SO4 ion detection is essential for accurate tissue analysis.

    Purpose of the Study:

    • To analytically study the benzidine technique for histochemical SO4 ion detection.
    • To establish experimental conditions for specific SO4 ion precipitation.
    • To develop an improved benzidine histochemical technique for SO4 detection, free from PO4 interference.

    Main Methods:

    • Analytical study of the benzidine technique using filter paper strips with sulfate-containing and sulfate-free solutions.
    • Investigation of benzidine solution pH influence on SO4 and PO4 ion precipitation.
    • Development and proposal of a modified benzidine histochemical technique for tissue sections.

    Main Results:

    • The influence of pH on SO4 and PO4 ion precipitation by benzidine was analyzed.
    • Specific experimental conditions for SO4 ion precipitation were successfully established.
    • A novel benzidine histochemical technique demonstrating high specificity for SO4 ions was developed.

    Conclusions:

    • The proposed benzidine histochemical technique effectively detects SO4 ions without PO4 interference.
    • This new technique offers improved specificity compared to previously proposed benzidine methods.
    • The optimized method is suitable for accurate histochemical analysis of SO4 ions in tissue sections.

    Related Experiment Videos