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STAR Protocols|December 23, 2025
Protocol for marker-free genome editing in Saccharomyces cerevisiae using universal donor templates and multiplexed CRISPR-Cas9Darshi Hemani, James H Grissom, Richard J ChiSTAR Protocols|December 28, 2025
Protocol to develop a preclinical severe contusive-compressive SCI mouse model for translational researchSissi Dolci, Marzia Di Chio, Giuseppe Busetto, et al.STAR Protocols|December 28, 2025
Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexingKevin Bigott, Victoria H Schoppel, Manuel Martinez-Osuna, et al.STAR Protocols|December 20, 2025
Protocol for assessing affective responses in caregiver-infant dyads during the still-face procedure using infrared thermal imagingNicolás Hinrichs, Sofia Attolini, Mirko Morgese Zangrandi, et al.STAR Protocols|January 8, 2026
Protocol for determining gut microbiota metabolites as substrates in mouse metabolismHan Fang, Dana Kukje Zada, Nicole G Barra, et al.STAR Protocols|December 16, 2025
Mass spectrometry protocol for tear fluid proteome profiling and biomarker discoveryKirstine Juul-Elbaek, Maria Peiris-Pagès, Vyacheslav Akimov, et al.STAR Protocols|December 20, 2025
Protocol for isolation of lipid droplets and mitochondria from acute myeloid leukemia cells for lipidomic analysisRan Zhao, Liang Zhang, Shuxin Dong, et al.STAR Protocols|December 20, 2025
Protocol for measuring in vivo, ex vivo, and in vitro glucose uptake in brown adipocytesChul-Hong Park, Ji Suk ChangSTAR Protocols|December 19, 2025
Protocol for generating native and unmodified bacterial RNA for nanopore direct RNA sequencing and downstream analysisZhihao Guo, Yanwen Shao, Lu Tan, et al.STAR Protocols|December 16, 2025
Protocol for establishment of parabiotic mouse models and validation by flow cytometric analysis of immune cellsJiahui Song, Wubin Zhao, Junkai Zhang, et al.Pageof 479